| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.078152 |
| Chromosome: | chromosome 6 |
| Location: | 7158353 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g297650 | UBC36 | (1 of 1) K10582 - ubiquitin-conjugating enzyme E2 Q (UBE2Q); E2 ubiquitin conjugating enzyme | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCTGCTTCACCACCACTGCGCCGCTGCGT |
| Internal bar code: | AAAGGGGGGGCACGCGGGCTGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 254 |
| LEAP-Seq percent confirming: | 97.723 |
| LEAP-Seq n confirming: | 1030 |
| LEAP-Seq n nonconfirming: | 24 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGTCCAAACATTAGAGCCCC |
| Suggested primer 2: | TAGACAGGGTGTAGGCCAGG |