| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.078157 |
| Chromosome: | chromosome 17 |
| Location: | 3209262 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g722250 | RRM14 | Putative rRNA (guanine-N2-)-methyltransferase; (1 of 1) 2.1.1.173//2.1.1.264 - 23S rRNA (guanine(2445)-N(2))-methyltransferase // 23S rRNA (guanine(2069)-N(7))-methyltransferase / 23S rRNA m(7)G(2069) methyltransferase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGTTTTTGTAAATATGCCCCCGTACATCCC |
| Internal bar code: | GCGGACGGTGCCAGATTTCACG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 487 |
| LEAP-Seq percent confirming: | 99.7753 |
| LEAP-Seq n confirming: | 1776 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATCGGATTGGCAAACTGAAG |
| Suggested primer 2: | CAGCCCTACCGTTGTGTTTT |