| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.078166 |
| Chromosome: | chromosome 12 |
| Location: | 5128539 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g527050 | (1 of 13) IPR001251//IPR011074 - CRAL-TRIO lipid binding domain // CRAL/TRIO, N-terminal domain | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGCAAGCTAGCACCTATTGCTGGACGCAGG |
| Internal bar code: | ACGTCCCGAGTCAGAGATCCAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 657 |
| LEAP-Seq percent confirming: | 98.9448 |
| LEAP-Seq n confirming: | 11158 |
| LEAP-Seq n nonconfirming: | 119 |
| LEAP-Seq n unique pos: | 70 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATTGATGGTTGCAGTGGTGA |
| Suggested primer 2: | GGTGTCAGGTGTCGGCTATT |