Insertion junction: LMJ.RY0402.078247_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.078247
Chromosome:chromosome 3
Location:4298606
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre03.g174672 (1 of 1) 3.1.1.93 - Mycophenolic acid acyl-glucuronide esterase / Mycophenolic acid acyl-glucuronide deglucuronidase CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CACGCTCGCCCTGTGGAGGCGCCAAACCTG
Internal bar code:GAGTGGCTGAGAGAGGGGGTCG

Confirmation - LEAP-Seq

LEAP-Seq distance:57
LEAP-Seq percent confirming:76.1668
LEAP-Seq n confirming:767
LEAP-Seq n nonconfirming:240
LEAP-Seq n unique pos:6

Suggested primers for confirmation by PCR

Suggested primer 1:ACGGCCTTGTGTGAAGTAGG
Suggested primer 2:AAATACCGCAGCGAGCTAGA