Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | + |
Strain: | LMJ.RY0402.078247 |
Chromosome: | chromosome 3 |
Location: | 4298606 |
Confidence (%): | 58 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre03.g174672 | (1 of 1) 3.1.1.93 - Mycophenolic acid acyl-glucuronide esterase / Mycophenolic acid acyl-glucuronide deglucuronidase | CDS |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CACGCTCGCCCTGTGGAGGCGCCAAACCTG |
Internal bar code: | GAGTGGCTGAGAGAGGGGGTCG |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 57 |
LEAP-Seq percent confirming: | 76.1668 |
LEAP-Seq n confirming: | 767 |
LEAP-Seq n nonconfirming: | 240 |
LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | ACGGCCTTGTGTGAAGTAGG |
Suggested primer 2: | AAATACCGCAGCGAGCTAGA |