| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.078255 |
| Chromosome: | chromosome 7 |
| Location: | 5706142 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g352650 | (1 of 2) PTHR23139//PTHR23139:SF58 - RNA-BINDING PROTEIN // SUBFAMILY NOT NAMED | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCAGCTGAAGCAAGTCATCACACTCGCCGC |
| Internal bar code: | GGGTGGCAGGCTGCACCGTATG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 138 |
| LEAP-Seq percent confirming: | 98.7453 |
| LEAP-Seq n confirming: | 787 |
| LEAP-Seq n nonconfirming: | 10 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCGTAGCAAGAAGGGACAAG |
| Suggested primer 2: | CTGCTGGAGAACGTGATGAA |