| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.078265 |
| Chromosome: | chromosome 8 |
| Location: | 823409 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g360850 | (1 of 1) K18798 - protein AFG1 (AFG1, LACE1) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCCCAATCCCGTGGTCCGGCCACACCCACC |
| Internal bar code: | TAAGGGACTGTGATTGAGATTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 932 |
| LEAP-Seq percent confirming: | 89.4737 |
| LEAP-Seq n confirming: | 221 |
| LEAP-Seq n nonconfirming: | 26 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACGGTCCATGATATCCCAAA |
| Suggested primer 2: | TAGCAGCAGGAGAGAAGGGA |