| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.078266 |
| Chromosome: | chromosome 9 |
| Location: | 6511212 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g407550 | (1 of 1) IPR000104//IPR003034 - Antifreeze protein, type I // SAP domain | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCAAAGAGCACAAGCGCGGGAAAAGTTGCT |
| Internal bar code: | TCTTCCCGGCATTGCTTGGTGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 764 |
| LEAP-Seq percent confirming: | 94.5817 |
| LEAP-Seq n confirming: | 3980 |
| LEAP-Seq n nonconfirming: | 228 |
| LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTCTTTGCGCTCTGTTTTCC |
| Suggested primer 2: | GACGCTAAGCAGGTACAGCC |