| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.078274 |
| Chromosome: | chromosome 13 |
| Location: | 3420650 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g587300 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTGAGTTGCTTGTTGTTCCTTGAATTGCTG |
| Internal bar code: | TGCTGCACCGTACGACTCCCCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1158 |
| LEAP-Seq percent confirming: | 99.5569 |
| LEAP-Seq n confirming: | 4494 |
| LEAP-Seq n nonconfirming: | 20 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATGAGAACTCGGCCTTCAGA |
| Suggested primer 2: | ACTGTTGGCTTGGGATCAAC |