Insertion junction: LMJ.RY0402.078286_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.078286
Chromosome:chromosome 13
Location:1810372
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre13.g575101 (1 of 726) IPR011009 - Protein kinase-like domain 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTCCCCAACATACTAACACGCTGCGTCGTG
Internal bar code:GAGTCCTGCACGTGGTGCGACG

Confirmation - LEAP-Seq

LEAP-Seq distance:454
LEAP-Seq percent confirming:45.6853
LEAP-Seq n confirming:810
LEAP-Seq n nonconfirming:963
LEAP-Seq n unique pos:3

Suggested primers for confirmation by PCR

Suggested primer 1:AACAACGCCCAGCTTTCTTA
Suggested primer 2:GCTGCTAGACTTCGGTTGGT