| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.078287 |
| Chromosome: | chromosome 5 |
| Location: | 386394 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g232950 | (1 of 1) K13110 - microfibrillar-associated protein 1 (MFAP1) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTGCCCCCTCTTTGGATGTCGGCACTTCAT |
| Internal bar code: | CGGTAATGGGTTGCGCCGGCGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 256 |
| LEAP-Seq percent confirming: | 96.1234 |
| LEAP-Seq n confirming: | 3645 |
| LEAP-Seq n nonconfirming: | 147 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAACTAAACCAAAACCGGGG |
| Suggested primer 2: | CCTCCACACACACACACACA |