| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.078314 |
| Chromosome: | chromosome 9 |
| Location: | 7477466 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g413750 | ABT1 | Putative aminotransferase; (1 of 1) 2.6.1.48 - 5-aminovalerate transaminase / Delta-aminovalerate transaminase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATGCACTCACATCCAGTGGCCCCCCGCAGC |
| Internal bar code: | GCGATGCGTCGGGCGAGGGGAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 640 |
| LEAP-Seq percent confirming: | 98.7662 |
| LEAP-Seq n confirming: | 6324 |
| LEAP-Seq n nonconfirming: | 79 |
| LEAP-Seq n unique pos: | 30 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTGTTGATGACGTCGATGGT |
| Suggested primer 2: | ATTTACTATCCACGCCACGC |