| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.078315 |
| Chromosome: | chromosome 12 |
| Location: | 7290083 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g557650 | (1 of 100) IPR001810 - F-box domain | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGAGAGAAGGTGCTCGAAAGGTGCGGTGTC |
| Internal bar code: | TTTACCCGTGTATAAGCAACTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 531 |
| LEAP-Seq percent confirming: | 99.0623 |
| LEAP-Seq n confirming: | 1796 |
| LEAP-Seq n nonconfirming: | 17 |
| LEAP-Seq n unique pos: | 22 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCACTACTGCTGCTGCTGAC |
| Suggested primer 2: | GATGGAGTTTTGCTTCTCGC |