Insertion junction: LMJ.RY0402.078328_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.078328
Chromosome:chromosome 4
Location:110907
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre04.g216700 PHOX,PHO5 Phosphate-repressible alkaline phosphatase; (1 of 2) 3.1.3.1 - Alkaline phosphatase / Phosphomonoesterase CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCAACGTCATGTGGCAGTACGACGTGAGTG
Internal bar code:AAGTGTGTCGGTGGCTACCTGA

Confirmation - LEAP-Seq

LEAP-Seq distance:671
LEAP-Seq percent confirming:97.4265
LEAP-Seq n confirming:265
LEAP-Seq n nonconfirming:7
LEAP-Seq n unique pos:3

Suggested primers for confirmation by PCR

Suggested primer 1:GCTGAACAGGTAGGAGTCGC
Suggested primer 2:TTCTGCTCTTGTGGTGTTGC