| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.078360 |
| Chromosome: | chromosome 3 |
| Location: | 2827958 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g162366 | (1 of 2) 1.10.3.3 - L-ascorbate oxidase / Ascorbase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGAGGTTGTGTGCGGGTTGAAGTGAGGGAT |
| Internal bar code: | TCCGGGGGTGCAGACGCGTAGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 26 |
| LEAP-Seq percent confirming: | 89.6359 |
| LEAP-Seq n confirming: | 8960 |
| LEAP-Seq n nonconfirming: | 1036 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAGTGAGTGGGTGTGAATGG |
| Suggested primer 2: | ACCATCGCTAGATCCACCAC |