| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.078367 |
| Chromosome: | chromosome 6 |
| Location: | 5557567 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g285350 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATAACCCATTGTGGGGAAGCAACACCCCTG |
| Internal bar code: | TGCCATGTTGGACACCAGAGGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 483 |
| LEAP-Seq percent confirming: | 40.439 |
| LEAP-Seq n confirming: | 1216 |
| LEAP-Seq n nonconfirming: | 1791 |
| LEAP-Seq n unique pos: | 18 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TACCGTATCCGCGTACATGA |
| Suggested primer 2: | CTCTACGGTGGATTCCGTGT |