| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.078403 |
| Chromosome: | chromosome 9 |
| Location: | 12229 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g386113 | (1 of 2) K01408 - insulysin (IDE, ide) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGTGCTGATGTGCCTGCCCCACGTCGGCAC |
| Internal bar code: | GCCCCAAGGGTAGCCGAAGGCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 246 |
| LEAP-Seq percent confirming: | 95.4122 |
| LEAP-Seq n confirming: | 2870 |
| LEAP-Seq n nonconfirming: | 138 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCCAGTGTGAGATGATGTGC |
| Suggested primer 2: | TTGTGGTTCATGTGGCAAGT |