Insertion junction: LMJ.RY0402.078444_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.078444
Chromosome:chromosome 2
Location:4948836
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre02.g103800 (1 of 31) IPR003590 - Leucine-rich repeat, ribonuclease inhibitor subtype intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CATTTTCATATGGGGATGCGAATACCGACG
Internal bar code:TACAGTAGCGCTGGTATGTGTC

Confirmation - LEAP-Seq

LEAP-Seq distance:758
LEAP-Seq percent confirming:98.4702
LEAP-Seq n confirming:4184
LEAP-Seq n nonconfirming:65
LEAP-Seq n unique pos:27

Suggested primers for confirmation by PCR

Suggested primer 1:ACGCGTCAAACTGAACACAG
Suggested primer 2:TACTTGCATACGGGTCTCCC