Insertion cassette: | CIB1 |
Side of cassette: | 5' |
Strand: | - |
Strain: | LMJ.RY0402.078448 |
Chromosome: | chromosome_13 |
Location: | 1616885 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre13.g573500 | FAP95 | Flagellar Associated Protein | antisense | CDS |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | TCACAAGTGGCGAGCGCGACAGGGCGGACA |
Internal bar code: | GGTCCTTTATGGGATTGAGGTT |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 1282 |
LEAP-Seq percent confirming: | 99.6317 |
LEAP-Seq n confirming: | 12443 |
LEAP-Seq n nonconfirming: | 46 |
LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | CACCACTCCGACGGTAACTT |
Suggested primer 2: | AGACACAGAGGGAGGCTCAA |