| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.078485 |
| Chromosome: | chromosome 9 |
| Location: | 1077970 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g400500 | (1 of 3) PF14494 - Domain of unknown function (DUF4436) (DUF4436) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCGAATGGGTACAGGTAGAGGCTGGTGGTG |
| Internal bar code: | CCTACCTCTCGGGGGACCCACT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 889 |
| LEAP-Seq percent confirming: | 99.4643 |
| LEAP-Seq n confirming: | 557 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGCCTTGTGGTGCATGTAAT |
| Suggested primer 2: | GTTGAGGACGTTTCGGTTGT |