| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.078535 |
| Chromosome: | chromosome 1 |
| Location: | 513399 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g002861 | SRR24 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGTAGGGCGGCCTTGCCTTTATGTGTTGGG |
| Internal bar code: | CTCTGAGCGGTAGTTCCTGGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 453 |
| LEAP-Seq percent confirming: | 98.0148 |
| LEAP-Seq n confirming: | 3851 |
| LEAP-Seq n nonconfirming: | 78 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGTTCTCCATCAACAACCGC |
| Suggested primer 2: | AGCAGTATGCAGGCAGGAGT |