Insertion junction: LMJ.RY0402.078539_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.078539
Chromosome:chromosome 5
Location:3344625
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre05.g240700 FAP378 Flagellar Associated Protein 378; (1 of 1) PF00530//PF05572 - Scavenger receptor cysteine-rich domain (SRCR) // Pregnancy-associated plasma protein-A (Peptidase_M43) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTCGATCCAAACCGCCCCCACGCTCCACGC
Internal bar code:GATAGGCGGGTCAGATACAACA

Confirmation - LEAP-Seq

LEAP-Seq distance:479
LEAP-Seq percent confirming:81.4112
LEAP-Seq n confirming:2873
LEAP-Seq n nonconfirming:656
LEAP-Seq n unique pos:22

Suggested primers for confirmation by PCR

Suggested primer 1:TTTACTCGGAGCCATGAACC
Suggested primer 2:AGAGGGTGGAAGGTGTGTTG