| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.078553 |
| Chromosome: | chromosome 16 |
| Location: | 3600433 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g683437 | (1 of 1) K16278 - E3 ubiquitin-protein ligase HOS1 [EC:6.3.2.19] (HOS1) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGCTTGGTCTCTCGTCGGCTTTGGCCTCAT |
| Internal bar code: | GGGGGTCCTGTTCGTCTCTGTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 534 |
| LEAP-Seq percent confirming: | 92.9204 |
| LEAP-Seq n confirming: | 105 |
| LEAP-Seq n nonconfirming: | 8 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GATGGTGATGGGGACATAGG |
| Suggested primer 2: | GTTGCATGGTGGCCTTAACT |