Insertion cassette: | CIB1 |
Side of cassette: | 5' |
Strand: | + |
Strain: | LMJ.RY0402.078560 |
Chromosome: | chromosome 6 |
Location: | 3474854 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
intergenic |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGCATCCGTGCCGTGCCTACAGTCTTGTCC |
Internal bar code: | GCTCGTGGGTTGTGCCACGTCG |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 433 |
LEAP-Seq percent confirming: | 98.6784 |
LEAP-Seq n confirming: | 224 |
LEAP-Seq n nonconfirming: | 3 |
LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | GGCTCCAAACATGGTTGTCT |
Suggested primer 2: | CTCACTGAGGAAGCCGTAGC |