| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.078588 |
| Chromosome: | chromosome 7 |
| Location: | 5531859 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g351300 | (1 of 2) PTHR11451//PTHR11451:SF41 - TRNA SYNTHETASE-RELATED // GLUTAMATE--TRNA LIGASE, MITOCHONDRIAL-RELATED | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AAGCAAACACACTCGTGCGGCCCTTGTCGC |
| Internal bar code: | TACTCTCGGGTCGACGGTGCTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 579 |
| LEAP-Seq percent confirming: | 98.1089 |
| LEAP-Seq n confirming: | 2127 |
| LEAP-Seq n nonconfirming: | 41 |
| LEAP-Seq n unique pos: | 28 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTTGAGGGTCCTGGTTTGAG |
| Suggested primer 2: | TCGTACCCCATTCCTACTGC |