Insertion junction: LMJ.RY0402.078647_3

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.078647
Chromosome:chromosome 17
Location:7043215
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre17.g746747 CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCTCAGCGGATTCCTTGTGTCACCTGATGC
Internal bar code:CCGGACAGTAGGTCCCCTCTGT

Confirmation - LEAP-Seq

LEAP-Seq distance:327
LEAP-Seq percent confirming:99.5444
LEAP-Seq n confirming:437
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:4

Suggested primers for confirmation by PCR

Suggested primer 1:AGCGCAACCAAGTAAGGCTA
Suggested primer 2:TGGCACTTTGTTTCCTACCC