Insertion junction: LMJ.RY0402.078648_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.078648
Chromosome:chromosome 1
Location:2183960
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre01.g011950 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTGATGCGCAGGGGTGCGATTGTCCTCTGC
Internal bar code:TCTTGCTGGTCGGTCAGGATCG

Confirmation - LEAP-Seq

LEAP-Seq distance:664
LEAP-Seq percent confirming:99.003
LEAP-Seq n confirming:3277
LEAP-Seq n nonconfirming:33
LEAP-Seq n unique pos:23

Suggested primers for confirmation by PCR

Suggested primer 1:GAACGAATCATGGACGAGGT
Suggested primer 2:CCGGCCAGCTACTTCTACAC