| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.078670 |
| Chromosome: | chromosome 13 |
| Location: | 4162199 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g592000 | (1 of 19) K08850 - aurora kinase, other [EC:2.7.11.1] (AURKX) | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGAAATTCAAGGCAAAGGTGCAAGTTTCCC |
| Internal bar code: | GGCTGAAGACTTGACGAGAATG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 748 |
| LEAP-Seq percent confirming: | 96.1842 |
| LEAP-Seq n confirming: | 731 |
| LEAP-Seq n nonconfirming: | 29 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGTAAGGTTGGGAGGTGGAG |
| Suggested primer 2: | TTACGGTGGGATTTCGAGAC |