| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.078691 |
| Chromosome: | chromosome 16 |
| Location: | 3600563 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g683437 | (1 of 1) K16278 - E3 ubiquitin-protein ligase HOS1 [EC:6.3.2.19] (HOS1) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGTCCCAGCAACACACTGCCTCCCCGCTAT |
| Internal bar code: | TGAGCTTCGAGTGTGTTGGCCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 388 |
| LEAP-Seq percent confirming: | 91.0122 |
| LEAP-Seq n confirming: | 3129 |
| LEAP-Seq n nonconfirming: | 309 |
| LEAP-Seq n unique pos: | 22 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GATGGTGATGGGGACATAGG |
| Suggested primer 2: | GTTGCATGGTGGCCTTAACT |