| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.078693 |
| Chromosome: | scaffold 52 |
| Location: | 5381 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre52.g761647 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGCGCACACACACTCGGGCGCACACACAT |
| Internal bar code: | GCGTCGCACGAATGAAAACACC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 167 |
| LEAP-Seq percent confirming: | 91.8224 |
| LEAP-Seq n confirming: | 393 |
| LEAP-Seq n nonconfirming: | 35 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TATGAGGAGGCGTATGGGAG |
| Suggested primer 2: | GCAGGGACAAGACAGTAGGC |