Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | - |
Strain: | LMJ.RY0402.078739 |
Chromosome: | chromosome_14 |
Location: | 3377550 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre14.g630200 | sense | intron |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | TTAAGGAAGTAGTAGACGGTGGGGGAGGGG |
Internal bar code: | AGTGTAAACGTGAGCACGTGGG |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 42 |
LEAP-Seq percent confirming: | 80.0 |
LEAP-Seq n confirming: | 56 |
LEAP-Seq n nonconfirming: | 14 |
LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | ACACGTTTTATCCACGGCTC |
Suggested primer 2: | ACTCGTTCAGGATCACCACC |