| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.078748 |
| Chromosome: | chromosome 9 |
| Location: | 3685331 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g390801 | (1 of 1) PTHR11918//PTHR11918:SF77 - RADICAL SAM PROTEINS // SUBFAMILY NOT NAMED | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGGGTCCATTGACACGGTATGTAGGTCAGC |
| Internal bar code: | GGAGTCTAGTCGAAAGTCCGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 89 |
| LEAP-Seq percent confirming: | 97.5806 |
| LEAP-Seq n confirming: | 121 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCGCGTTCATCTCACTTGTA |
| Suggested primer 2: | AGAGGGAGTACAATGGGCCT |