| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.078769 |
| Chromosome: | chromosome 3 |
| Location: | 4033461 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g172250 | (1 of 1) PTHR11649:SF5 - GTP-BINDING PROTEIN | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGGGAGTGGCAGCAGCGGTGCCGGCAGTAT |
| Internal bar code: | TTGTATGCACTGCCCGGCAAAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 282 |
| LEAP-Seq percent confirming: | 99.1803 |
| LEAP-Seq n confirming: | 242 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTGTTGTTGACTCCGCACAT |
| Suggested primer 2: | TGACAGGATGCGATTCTCAG |