| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.078794 |
| Chromosome: | chromosome 2 |
| Location: | 565023 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g077600 | FAP51 | Flagellar Associated Protein 51 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCGTCCACGCGGCGGGCCAGGTCCACGTAG |
| Internal bar code: | TTAACCGGAGGGACACGGTGTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 199 |
| LEAP-Seq percent confirming: | 68.4825 |
| LEAP-Seq n confirming: | 704 |
| LEAP-Seq n nonconfirming: | 324 |
| LEAP-Seq n unique pos: | 15 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATCCCCCTAATGGTGTCTCC |
| Suggested primer 2: | CTAGCGAAGTACAGGCAGGG |