| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.078825 |
| Chromosome: | chromosome 7 |
| Location: | 1040272 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g319550 | (1 of 1) PF10442 - FIST C domain (FIST_C) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGTTGCGCACGGGCTTGAGGTAGCTTCTCC |
| Internal bar code: | CCATTCTTTGCATGCTCCATAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 481 |
| LEAP-Seq percent confirming: | 89.7346 |
| LEAP-Seq n confirming: | 1285 |
| LEAP-Seq n nonconfirming: | 147 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGCTAGAGACATTTTTCGGC |
| Suggested primer 2: | AGGACTGAATGCGGTAATGG |