| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.078846 |
| Chromosome: | chromosome 1 |
| Location: | 3214728 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g020141 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGACCGGCCAGAGCATGCGTACGGCGTTGG |
| Internal bar code: | CGTGGAGAGGCTGTCCGGACCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 246 |
| LEAP-Seq percent confirming: | 97.4877 |
| LEAP-Seq n confirming: | 1591 |
| LEAP-Seq n nonconfirming: | 41 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TAGCACACTTTCCATGCTCG |
| Suggested primer 2: | TACCTATCCACGTGGTGCAA |