Insertion cassette: | CIB1 |
Side of cassette: | 5' |
Strand: | + |
Strain: | LMJ.RY0402.078847 |
Chromosome: | chromosome_11 |
Location: | 640258 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre11.g467616 | antisense | CDS |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | GACCACAGCATGCGGCCCGCACCAACGTCG |
Internal bar code: | ACGTTCGGCGTGATGACCTCAT |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 243 |
LEAP-Seq percent confirming: | 98.5437 |
LEAP-Seq n confirming: | 203 |
LEAP-Seq n nonconfirming: | 3 |
LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | ATAGCCATGCTCCCACACTC |
Suggested primer 2: | CGTGGTCATATGTCTGTGGC |