| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.078851 |
| Chromosome: | chromosome 14 |
| Location: | 1446336 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g617800 | (1 of 3) PTHR12385:SF44 - PLASMA-MEMBRANE CHOLINE TRANSPORTER FAMILY PROTEIN | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCGCTGTACCTGCTGCTGTTCCGACGCTAC |
| Internal bar code: | CTCAGAACGCCCCGAACTCCTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 810 |
| LEAP-Seq percent confirming: | 99.8368 |
| LEAP-Seq n confirming: | 4283 |
| LEAP-Seq n nonconfirming: | 7 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TAGTACACCACCGCACCGTA |
| Suggested primer 2: | TCATTATCTGGCGCAGAGTG |