Insertion junction: LMJ.RY0402.078913_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.078913
Chromosome:chromosome 1
Location:3815002
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre01.g024650 CTL1 carbohydrate-binding protein; (1 of 2) PF00059//PF07714//PF14295 - Lectin C-type domain (Lectin_C) // Protein tyrosine kinase (Pkinase_Tyr) // PAN domain (PAN_4) CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGCCATCGGGATCGTCCACGCAGTTATCGC
Internal bar code:GCTTAAGTAGGCGTCATGAGTG

Confirmation - LEAP-Seq

LEAP-Seq distance:193
LEAP-Seq percent confirming:99.0196
LEAP-Seq n confirming:101
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:2

Suggested primers for confirmation by PCR

Suggested primer 1:CAAACGTCACATCGGTTTTG
Suggested primer 2:CTCGTGCTTTAACCTGGAGC