Insertion junction: LMJ.RY0402.078934_3

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.078934
Chromosome:chromosome 6
Location:2841067
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre06.g271700 (1 of 2) 3.4.17.4 - Gly-Xaa carboxypeptidase / Glycine carboxypeptidase; Metallopeptidase M20 family protein intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCCGGCTGTGCTCACTTCCACTTCCTTGCA
Internal bar code:AATCGAGGCCGTTGCGACTGTA

Confirmation - LEAP-Seq

LEAP-Seq distance:359
LEAP-Seq percent confirming:99.4652
LEAP-Seq n confirming:1860
LEAP-Seq n nonconfirming:10
LEAP-Seq n unique pos:5

Suggested primers for confirmation by PCR

Suggested primer 1:ACCTCACACAGCAAGCACAC
Suggested primer 2:GGGTTACACCCAAAGCTGAA