| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.078956 |
| Chromosome: | chromosome 1 |
| Location: | 5422948 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g038100 | BES1 | (1 of 10) PF01062 - Bestrophin, RFP-TM, chloride channel (Bestrophin); putative chloride channel | CDS/intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GATAGGGCTTTCGCTGACGACGCGTAGAGC |
| Internal bar code: | CTTGCGGAGCGTTTCCAGCTCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 959 |
| LEAP-Seq percent confirming: | 99.6948 |
| LEAP-Seq n confirming: | 8819 |
| LEAP-Seq n nonconfirming: | 27 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGGGTTTACCGCAGATTGTG |
| Suggested primer 2: | TAGTCGGCGACATTTATCCC |