Insertion cassette: | CIB1 |
Side of cassette: | 5' |
Strand: | + |
Strain: | LMJ.RY0402.079063 |
Chromosome: | chromosome 7 |
Location: | 738643 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre07.g317750 | intron |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GAGAGGCGATTAGGTTGGGGTTGGGCTGGC |
Internal bar code: | CCCCTACGCTTCATGTATTAAC |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 1029 |
LEAP-Seq percent confirming: | 98.8125 |
LEAP-Seq n confirming: | 3162 |
LEAP-Seq n nonconfirming: | 38 |
LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | GGCAGGTGAGGTAATGTCGT |
Suggested primer 2: | CAGCAAGCCACACACTCCTA |