| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.079067 |
| Chromosome: | chromosome 16 |
| Location: | 5156443 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g683500 | HEL64 | (1 of 1) K14778 - ATP-dependent RNA helicase DDX49/DBP8 [EC:3.6.4.13] (DDX49, DBP8); DEAD/DEAH box helicase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCGTAGGCGTGTGGCGCACGTAGCATGAGG |
| Internal bar code: | GAGACTAACGCAGCACGGCGGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 814 |
| LEAP-Seq percent confirming: | 98.115 |
| LEAP-Seq n confirming: | 2082 |
| LEAP-Seq n nonconfirming: | 40 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTATTGTGCTTGGGAGGCAT |
| Suggested primer 2: | CCGACGGTTTCAAAACAACT |