Insertion junction: LMJ.RY0402.079110_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.079110
Chromosome:chromosome 7
Location:2324257
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre07.g327950 MAW9 Membrane-associated hydroxyproline-rich glycoprotein 9 CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CAACTCATGGCCTCGTTTGAGATCGCCAAG
Internal bar code:TATACAAACTGCATCTACCGAG

Confirmation - LEAP-Seq

LEAP-Seq distance:277
LEAP-Seq percent confirming:44.4444
LEAP-Seq n confirming:8
LEAP-Seq n nonconfirming:10
LEAP-Seq n unique pos:5

Suggested primers for confirmation by PCR

Suggested primer 1:CCTCGACTTCCCCTTCCTC
Suggested primer 2:TAGGTTTGGGTTTGGGTTGA