| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.079118 |
| Chromosome: | chromosome 14 |
| Location: | 3244179 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g629300 | (1 of 3) K11855 - ubiquitin carboxyl-terminal hydrolase 36/42 [EC:3.4.19.12] (USP36_42) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGTGGGGGCAAGGCTCCGTGTGCAACGCT |
| Internal bar code: | CACCTAGTTAGTTCCGAGCGCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1424 |
| LEAP-Seq percent confirming: | 99.4695 |
| LEAP-Seq n confirming: | 1125 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCACACAAGTGCATTTGACC |
| Suggested primer 2: | GATGGCTGGGATCAGGAGTA |