| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.079125 |
| Chromosome: | chromosome_9 |
| Location: | 4733071 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre09.g397142 | TRP15 | Transient receptor potential ion channel protein | antisense | intron |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | TGTGGGGTGCGCAGGTGAAGGCCAGGAGCA |
| Internal bar code: | CAAATGAAGACCGTTGTATTGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 134 |
| LEAP-Seq percent confirming: | 93.4884 |
| LEAP-Seq n confirming: | 201 |
| LEAP-Seq n nonconfirming: | 14 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGTGGGGTGTACTTGGAACA |
| Suggested primer 2: | ACACGCTTACACACGCTCAC |