| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.079128 |
| Chromosome: | chromosome 7 |
| Location: | 4129951 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g342750 | (1 of 1) K18106 - D-galacturonate reductase (GAAA) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACGGTGGCGGTCACGGCCATACTGCATGCG |
| Internal bar code: | TCCCCCGTATACCATACGGAAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 458 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 548 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCAACCAGTAGGAGAGCAGC |
| Suggested primer 2: | TGGTGGTGGTGAGGTAGACA |