Insertion junction: LMJ.RY0402.079128_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.079128
Chromosome:chromosome 16
Location:1694583
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre16.g654750 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCTGGCTAGTGTGCCCATCGTGCACGAGGG
Internal bar code:ACATAGGTTTTTTTCGCGGATT

Confirmation - LEAP-Seq

LEAP-Seq distance:312
LEAP-Seq percent confirming:99.4259
LEAP-Seq n confirming:1905
LEAP-Seq n nonconfirming:11
LEAP-Seq n unique pos:3

Suggested primers for confirmation by PCR

Suggested primer 1:GCTTGGAAGCAGTTTTCGAC
Suggested primer 2:AGTCCAGTCCAGCCAAAATG