| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.079134 |
| Chromosome: | chromosome 14 |
| Location: | 2610660 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g625950 | PAP4 | (1 of 1) 2.7.7.19//2.7.7.72 - Polynucleotide adenylyltransferase / RNA adenylating enzyme // CCA tRNA nucleotidyltransferase / tRNA-nucleotidyltransferase; Class-II RNA nucleotidyl transferase 4, mitochondrial | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTCCCAAAGAGACAGGTGGGGGGTAGCCAT |
| Internal bar code: | CTTGCCTTTGACCGGAGGTTCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 824 |
| LEAP-Seq percent confirming: | 99.5136 |
| LEAP-Seq n confirming: | 7366 |
| LEAP-Seq n nonconfirming: | 36 |
| LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCAGCCCAAACTAAACCAAA |
| Suggested primer 2: | CCAAAGCAAGCCAAACTAGC |