| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.079142 |
| Chromosome: | chromosome 14 |
| Location: | 1684699 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g619300 | CYN16 | Cyclophilin 16; (1 of 60) 5.2.1.8 - Peptidylprolyl isomerase / Rotamase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATAGTGTAGCGGCTTCTTGGACGACTTGCC |
| Internal bar code: | GTGCCTTCCGTCGCCACCGAGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 561 |
| LEAP-Seq percent confirming: | 99.4033 |
| LEAP-Seq n confirming: | 1999 |
| LEAP-Seq n nonconfirming: | 12 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGCGCATTTAACTAATGGGT |
| Suggested primer 2: | CTGGCTTAAAGCAGTGGGAG |