Insertion junction: LMJ.RY0402.079145_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.079145
Chromosome:chromosome 13
Location:1391526
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre13.g571902 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CAATTGTCATTGATTGCGACGACGCACCAA
Internal bar code:TAATGTTGTGGTTTTGGACGAA

Confirmation - LEAP-Seq

LEAP-Seq distance:613
LEAP-Seq percent confirming:99.7266
LEAP-Seq n confirming:1824
LEAP-Seq n nonconfirming:5
LEAP-Seq n unique pos:2

Suggested primers for confirmation by PCR

Suggested primer 1:GGCGACCTCGATGGTAGATA
Suggested primer 2:CAGAACGGGTTGTGATGATG